Time-restricted feeding mice a high-fat diet induces a unique lipidomic profile

Time-restricted feeding (TRF) can reduce adiposity and lessen the co-morbidities of obesity. Mice consuming obesogenic high-fat (HF) diets develop insulin resistance hepatic steatosis, but have elevated indices long-chain polyunsaturated fatty acids (LCPUFA) that may be beneficial. While TRF impacts lipid metabolism, scant data exist regarding impact upon lipidomic composition tissues. We (1) tested hypothesis a HF diet elevates LCPUFA while preventing steatosis (2) determined lipidome in plasma, liver, adipose tissue. For 12 weeks, male, adult mice were fed control ad libitum, libitum (HF-AL), or with TRF, hours during dark phase (HF-TRF). HF-TRF prevented resulting from by HF-AL treatment. TRF-blocked plasma increases induced treatment concentrations triacylglycerols non-esterified saturated acids. Analysis demonstrated did not elevate reducing steatosis. However, created separate signature for triacylglycerols, phosphatidylcholine, phosphatidylethanolamine species modified gene protein expression consistent reduced acid synthesis restoration diurnal signaling. increased content visceral In summary, alters profile tissue, creating third distinct metabolic state indicative positive adaptations following intake.